Galanthus Nivalis Lectin (GNL), Agarose bound

Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca⁺⁺ or Mn⁺⁺ for binding. Binding seems to be preferentially directed toward structures containing (?-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind ?-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is ?2-macroglobulin. GNL binds to many viral glycoproteins.

Agarose bound* Galanthus nivalis lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10⁷ daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

• Maximum carbohydrate binding activity of the coupled lectins is retained
• Linkage is stable over a range of pH values
• Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
• No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 100 mM - 200 mM ?-methylmannoside

*3 mg lectin/ml gel