Description
- SKU:
- B-1245-2
- Additional Names:
- Lectins & Glycobiology Reagents [Lectins ], Snowdrop lectin, Mannose binding lectin
- Application:
- Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT, FLUOROSPOT
- Conjugate:
- Biotin
- Extra Details:
- Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (?-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind ?-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is ?2-macroglobulin. GNL binds to many viral glycoproteins. Biotinylated Galanthus nivalis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: 100 mM - 200 mM ?-methylmannoside
- Purification:
- Affinity Purified
- Research Area:
- Glycobiology
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 2-8 [o]C; Store frozen for long term storage
- Supplier:
- Vector Laboratories
- Size:
- 2mg
- Manufacturer's Data Sheet:biotinylated-galanthus-nivalis-lectin-gnl.html
