Sambucus Nigra Lectin (SNA, EBL), Biotinylated

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SKU:

B-1305-2

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Description

SKU:: B-1305-2
Additional Names:: Lectins & Glycobiology Reagents [Lectins ], Elderberry Lectin, Sialic acid binding lectin
Application:: Immunohistochemistry, IHC-Frozen, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, ELISPOT, FLUOROSPOT
Conjugate:: Biotin
Extra Details:: Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in ?-2,6 and to a lesser degree, ?-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis.Biotinylated Sambucus nigra lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid
Purification:: Affinity Purified
Research Area:: Glycobiology
Shipping Conditions:: Ambient
Storage Conditions:: 2-8 [o]C; Store frozen for long term storage
Supplier:: Vector Laboratories
Size:: 2mg