Description
Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.
The kit is designed for the antioxidant capacity measurement in foods, beverages and biological samples.
Background: Bioquochem ABTS assay kit is recommended for total antioxidant activity of solutions of pure substances, aqueous mixtures and beverages. The assay described here involves the direct production of the blue/green ABTS•+ chromophore. This has absorption maxima at 734 nm. The addition of antioxidants to the pre-formed radical cation, reduces it ABTS depending on the antioxidant activity and the concentration of the antioxidant. In our assay a solution of ABTS at neutral pH and in the presence of a suitable solution, can form a stable and colored radical cation (ABTS•+) which shows a maximum of absorbance at 734 nm. Antioxidant compounds quench the color and produce a decoloration of the solution which is proportional to their amount. This reaction is rapid and the end point, which is stable, is taken as a measure of the antioxidative efficiency.
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