Anti-4R-Tau pAb (Rabbit, Antiserum)

Anti-4R-Tau pAb — prepared from rabbits immunized with synthetic N279D 4R-tau peptide [VQIIDKKLDLSNVQSKC] — recognizes 4R tau isoforms, regardless of deamidation as occurs extensively in AD brains. Validated for western blot and IHC(p), this isoform-specific tau antibody is useful for immunohistochemical and biochemical studies of tau species in diverse neurodegenerative diseases.

Tau is a microtubule-associated protein that assembles into insoluble, filamentous, and hyperphosphorylated inclusions in many neurodegenerative diseases. The adult human brain expresses 6 distinct splice forms divided into two groups depending on the inclusion of exon 10 to generate either 3-repeat (3R) or 4-repeat (4R) isoforms. Distinct tau isoforms accumulate in diseased brains: all 6 isoforms in AD, 3R tau in Pick’s disease, and 4R tau in PSP and CBD. Therefore, isoform-specific tau antibodies are useful tools for immunohistochemical and biochemical studies of tau species in diseased brains. Tau deposited in AD is extensively deamidated at N279. This anti-4R tau pAb specifically recognizes 4R tau isoforms regardless of deamidation and strongly stained tau in AD brain.

Specifications


• Product type                primary antibody

• Immunogen                 synthetic N279D 4R-tau peptide (VQIIDKKLDLSNVQSKC)

• Raised in                     rabbit

• Source                         anti-serum

• Form                           liquid with 0.1% NaN3 as a preservative

• Volume                       50 uL

• Label                           none

• Specificity                   unlabeled 4R-tau isoforms

• Cross reactivity           human, mouse, rat

• Storage                        below -20°C. (below -70°C for prolonged storage). Aliquot to avoid cycles of freeze/thaw.


Recommended dilutions


• Western blotting                     1/2000-1/5000

• Immunohistochemistry           1:3000-1/20000

• Other applications have not been tested.

• Optimal dilutions/concentrations should be determined by the end user.


References


1. Hasegawa M, Watanabe S, Kondo H, et al, 3R and 4R tau isoforms in paired helical filaments in Alzheimer's disease. Acta Neuropathol. 2013 Nov 9

2. Dan A, Takahashi M, Masuda-Suzukake M, et al.Extensive deamidation at asparagine residue 279 accounts for weak immunoreactivity of tau with RD4 antibody in Alzheimer's disease brain. Acta Neuropathol