Anti Autophagy-Related Protein 8i (ATG8i) pAb (Rabbit, Antiserum)

Programmed cell death (PCD) in plants is a crucial component of development and defense mechanisms. In animals, different types of cell death (apoptosis, autophagy, and necrosis) have been distinguished morphologically and discussed in these morphological terms. PCD is largely used to describe the processes of apoptosis and autophagy (although some use PCD and apoptosis interchangeably) while necrosis is generally described as a chaotic and uncontrolled mode of death. In plants, the term PCD is widely used to describe most instances of death observed. At present, there is a vast array of plant cell culture models and developmental systems being studied by different research groups and it is clear from what is described in this mass of literature that, as with animals, there does not appear to be just one type of PCD in plants. It is fundamentally important to be able to distinguish between different types of cell death for several reasons. For example, it is clear that, in cell culture systems, the window of time in which ‘PCD’ is studied by different groups varies hugely and this can have profound effects on the interpretation of data and complicates attempts to compare different researcher’s data. In addition, different types of PCD will probably have different regulators and modes of death. For this reason, in plant cell cultures an apoptotic-like PCD (AL-PCD) has been identified that is fairly rapid and results in a distinct corpse morphology which is visible 4–6 h after release of cytochrome and other apoptogenic proteins. This type of morphology, distinct from autophagy and from necrosis, has also been observed in examples of plant development. [from: Reape TJ, Molony EM, and McCabe PF. (2008) Programmed cell death in plants: distinguishing between different modes. Journal of Experimental Botany. 59(3): 435–444.]

ATG8 is an autophagy-related gene, a homolog protein of LC3. ATG8 is conjugated with phosphatidyl ethanol amine on its COOH terminal glycine via the ATG7 and ATG3 E1-like enzyme system. Higher plants have multiple ATG8 homologs comprising two subfamilies, ATG8a-g and ATG8h-i in Arabidopsis. Anti-soybean ATG8i antibody was raised against GST-fused GmATG8i and cross reacts with plant ATG8h- and ATG8i-related proteins, but not with plant ATG8a-g.

References:
1) Nang MPSH, Tanigawa H, Ishibashi Y, Zheng SH, Yuasa T and Iwaya-Inoue M. (2009) Nutrient starvation differentially regulates GmATG8i in soybean seedlings. Plant Biotechnol. 26:317-326.
2) Okuda M, Nang MP, Oshima K, Ishibashi Y, Zheng SH, Yuasa T, Iwaya-Inoue M. (2011) Ethylene signal mediates induction of GmATG8i in soybean plants under starvation stress, Biosci Biotechnol Biochem. 75(7):1408-12. PMID : 21737912.