Description
Application: WB, IF, ICC
Clonality: Polyclonal
Host: Rabbit
Purification: Purified - Affinity
Reactivity: Mouse, Human
EDD / UBR5 (2,799 aa, 309 kDa) is E3 ubiquitin-protein ligase which is a component of the N-end rule
pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to
the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or
transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle
progression. May havetumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the
DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also
acts as a regulator of DNA damage response byacting as a suppressor of RNF168, an E3 ubiquitin-protein ligase
that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a
guard against excessive spreading of ubiquitinated chromatin at damagedchromosomes. Application: 1) Western blotting (1/1,000 dilution) (Fig. 1)
Not tested for other applications.
Immunogen: Synthetic peptide of human EDD protein corresponding to 394-408 amino acids,
C-KWSESEPYRNAQNPS, conjugated with KLH
Cross reactivity: Human (HeLa, HEK293T, MCF7) and mouse (NIH3T3). Note that expression level of EDD varies
greatly, depending on tissues and cell types (see Fig.1).
Not tested in other species.
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Clonality: Polyclonal
Host: Rabbit
Purification: Purified - Affinity
Reactivity: Mouse, Human
EDD / UBR5 (2,799 aa, 309 kDa) is E3 ubiquitin-protein ligase which is a component of the N-end rule
pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to
the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or
transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle
progression. May havetumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the
DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also
acts as a regulator of DNA damage response byacting as a suppressor of RNF168, an E3 ubiquitin-protein ligase
that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a
guard against excessive spreading of ubiquitinated chromatin at damagedchromosomes. Application: 1) Western blotting (1/1,000 dilution) (Fig. 1)
Not tested for other applications.
Immunogen: Synthetic peptide of human EDD protein corresponding to 394-408 amino acids,
C-KWSESEPYRNAQNPS, conjugated with KLH
Cross reactivity: Human (HeLa, HEK293T, MCF7) and mouse (NIH3T3). Note that expression level of EDD varies
greatly, depending on tissues and cell types (see Fig.1).
Not tested in other species.
