Description
Application: WB
Clonality: Polyclonal
Host: Rabbit
Purification: Serum
General information: Each antibody was raised in rabbits against the corresponding synthetic peptide or the purified enzyme. Therefore, Protein A or
anti-(rabbit IgG) antibodies can be used as the second antibodies for immunoblotting.
Sample treatment: Please do not boil the protein or membrane samples for immunoblotting. We recommend the treatment at 70ðC for 10 min.
Sample amount: For the major component of membranes, such as acuolar and plasma membrane aquaporins (TIPsand PIPs) and H+-pyrophosphatase, a small amount of the crude membrane fraction (a few micrograms) is enough to detect the antigen protein. When you use the purified preparation of vacuolar and plasma membranes, the antibodies can detect the antigenprotein in one microgram membrane preparation.
Antibody dilution: Please dilute the antibody solution to 1:2000 or more with TBS (Tris-buffered saline) or PBS (phosphate-buffered saline), if you use the ECL
detection system. For example, 5 uL of the original antibody solution can be diluted with 10 mL of TBS. The diluted antibody solutions can be used twice or three times within a month. In this case, please add sodium azide into the antibody solution at 0.05% before the first experiment and store at 20ðC or 80ðC.
Second antibodies: We recommend HRP-linkd Protein A and HRP-linked anti-(rabbit IgG) antibodies. Please dilute the second antibody to 1:3000 with TBS or PBS.
Antigen detection: We recommend the ECL detection system for immunoblotting, because its sensitivity is high and you can select the best expose period for
detection of the antigen.
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Clonality: Polyclonal
Host: Rabbit
Purification: Serum
General information: Each antibody was raised in rabbits against the corresponding synthetic peptide or the purified enzyme. Therefore, Protein A or
anti-(rabbit IgG) antibodies can be used as the second antibodies for immunoblotting.
Sample treatment: Please do not boil the protein or membrane samples for immunoblotting. We recommend the treatment at 70ðC for 10 min.
Sample amount: For the major component of membranes, such as acuolar and plasma membrane aquaporins (TIPsand PIPs) and H+-pyrophosphatase, a small amount of the crude membrane fraction (a few micrograms) is enough to detect the antigen protein. When you use the purified preparation of vacuolar and plasma membranes, the antibodies can detect the antigenprotein in one microgram membrane preparation.
Antibody dilution: Please dilute the antibody solution to 1:2000 or more with TBS (Tris-buffered saline) or PBS (phosphate-buffered saline), if you use the ECL
detection system. For example, 5 uL of the original antibody solution can be diluted with 10 mL of TBS. The diluted antibody solutions can be used twice or three times within a month. In this case, please add sodium azide into the antibody solution at 0.05% before the first experiment and store at 20ðC or 80ðC.
Second antibodies: We recommend HRP-linkd Protein A and HRP-linked anti-(rabbit IgG) antibodies. Please dilute the second antibody to 1:3000 with TBS or PBS.
Antigen detection: We recommend the ECL detection system for immunoblotting, because its sensitivity is high and you can select the best expose period for
detection of the antigen.
