Anti Influenza B Virus Hemagglutinin (HA) mAb (Clone 10B8)

Application: WB, IF, ICC, IP, Neutralization, ELISA, Immuno-inhibition 
Clonality: Monoclonal 
Host: Mouse 
Purification: IgG 
Reactivity: Influenza B Virus 
Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Application: 1) Western blotting (1/500~1/1,000 dilution)

2) Immunofluorescent and Immunocytochemical staining (1/100~1/200 dilution)

3) Immunoprecipitation (1/200 dilution)

4) Neutralization of infectivity (NT) (assay dependent)

5) Hemagglutination Inhibition (HI) (assay dependent)

6) ELISA (assay dependent)

Specificity: According to Ref.1 during epidemic in Osaka 1996-97, 10B8 antibody reacted with HA protein of all Influenza B virus isolates belonging to Victoria group tested (73 strains) and none of clinical 27 isolates belonging to Yamagata group as examined by PAP staining. Italso reacts with Victoria group vaccine strains; Shangdong/7/1997, Malasia/2506/2004. However, note that HA changes during passages and may change reactivity to this antibody. By western blotting, reactivity with B/Malasia/2506/2004 and B/Massachusetts/2/2012 was tested positive.

No cross reactivity with any strains of influenza A viruse.