Description
Clonality: Monoclonal
Host: Mouse
Purification: Supernatant
Reactivity: Human
Nidogen-1 (originally also named entactin) and its homologue nidogen-2 are ubiquitous and highly conserved components of basement membranes (BMs), though nidogen-2 shows more restricted expression patterns throughout development and some tissue specificity in the adult [1-5]. In BMs nidogens play a fundamental role in linking the collagen type IV-laminins networks and in bridging these networks with oligomers of perlecan, matrilins and fibulins. Nidogen-1 is encoded by the NID1 gene and is translated as a 158 kDa globular protein containing three distinct globules with diverse ligand binding specificity. Gene deletion of either nidogen does not disrupt developmental BM assembly, whereas ablation of both NID1 and NID2 murine genes leads to BM abnormalities and prenatal death [6-8]. Alterations in nidogen-1 expression have been associated with neoplastic transformation (e.g. hepatocarcinoma, melanoma, bladder carcinoma, renal carcinoma, grastrointestinal cancer), skin, renal, retinal, muscle and connective tissue degenerative diseases.
References:
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6) Murshed M, et all., Mol Cell Biol. 2000 Sep;20(18):7007-12. PMID: 10958695
7) Miosge N, et all., Matrix Biol. 2002 Nov;21(7):611-21. PMID: 12475645
8) Bader BL, et all., Mol Cell Biol. 2005 Aug;25(15):6846-56. PMID: 16024816
