Description
Application: ICC, IF, WB
Clonality: Monoclonal
Host: Mouse
Purification: IgG
Reactivity: S. cerevisiae, Human, S. pombe, Tetrahymena
Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly
embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and
cytoplasmic side of NPC.This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid
repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In
addition, Nup98 plays roles in several important biological events such as gene expression, mitotic checkpoint, and
pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal
translocation.
In ciliates, a unicellular organism having two functionally distinct nuclei, GLFG-Nup98 is present in one of the
nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these different Nup98s participate in a
nucleus-selective transport mechanism. Application: 1) Western blotting, (S. cerevisiae, S. pombe,) Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, Tetrahymena, S. cerevisiae, S. pombe.
Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of
Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664
Epitope determined: GLF (Ref.1)
Reactivity: Human, S. cerevisiae, S. pombe and Tetrahymena. Not tested in other species
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Clonality: Monoclonal
Host: Mouse
Purification: IgG
Reactivity: S. cerevisiae, Human, S. pombe, Tetrahymena
Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly
embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and
cytoplasmic side of NPC.This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid
repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In
addition, Nup98 plays roles in several important biological events such as gene expression, mitotic checkpoint, and
pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal
translocation.
In ciliates, a unicellular organism having two functionally distinct nuclei, GLFG-Nup98 is present in one of the
nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these different Nup98s participate in a
nucleus-selective transport mechanism. Application: 1) Western blotting, (S. cerevisiae, S. pombe,) Not suitable for human. Clone 13C2 is recommended for WB in human Tetrahymena and yeasts.
2) Most suitable for Immunofluorescence staining / Immunocytochemistry for human, Tetrahymena, S. cerevisiae, S. pombe.
Immunogen: Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of
Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664
Epitope determined: GLF (Ref.1)
Reactivity: Human, S. cerevisiae, S. pombe and Tetrahymena. Not tested in other species
