Description
Application: Neutralization, IP, DB, ELISA, WB
Clonality: Polyclonal
Host: Rabbit
Purification: Serum
Reactivity: Bordetella pertussis
Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the
ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and
prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26
kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa). This product was highly purified
(>90% pure) from Bordetellapertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was
confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below). Application: 1. Western blotting (1/2,000~1/10,000 dilution)
2. ELISA (1/10,000~1/20,000 dilution)
3. Dot blotting (1/2,000~1/10,000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralising (Assay dependent)
Other applications have not been tested.
Immunogen: Immunization was Initiated with toxoid and boosted with native toxin (BioAcademia 01-503)
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Clonality: Polyclonal
Host: Rabbit
Purification: Serum
Reactivity: Bordetella pertussis
Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the
ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and
prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26
kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa). This product was highly purified
(>90% pure) from Bordetellapertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was
confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below). Application: 1. Western blotting (1/2,000~1/10,000 dilution)
2. ELISA (1/10,000~1/20,000 dilution)
3. Dot blotting (1/2,000~1/10,000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralising (Assay dependent)
Other applications have not been tested.
Immunogen: Immunization was Initiated with toxoid and boosted with native toxin (BioAcademia 01-503)
