Description
Clonality: Monoclonal
Host: Mouse
Purification: Ig-PG
Puromycin is an aminonucleoside antibiotic derived from the bacterial strain Streptomyces alboniger. Structurally resembling the 3' end of aminoacyl-tRNAs, it can bind to translating ribosomes and cause premature chain termination. Classically, protein biosynthesis has been monitored using pulse-chase and flooding dose methods which both employ radioactive amino acid labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling and allows for the direct evaluation/quantification of translation using standard immunochemical methods.
Features:
1) Easy to evaluate and quantify protein biosynthesis
2) An alternative to the traditional pulse-chase method that relies on radioactive amino acids
3) Applicable to Western blot and ELISA analyses
References:
1) Kelleher AR, Gordon BS, Kimball SR, Jefferson LS. Changes in REDD1, REDD2, and atrogene mRNA expression are prevented in skeletal muscle fixed in a stretched position during hindlimb immobilization. Physiol Rep. 2014 Feb 25;2(2):e00246. doi: 10.1002/phy2.246. eCollection 2014 Feb 1. PubMed PMID: 24744910; PubMed Central PMCID: PMC3966240.
2) Kelleher AR, Kimball SR, Dennis MD, Schilder RJ, Jefferson LS. The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb. Am J Physiol Endocrinol Metab. 2013 Jan 15;304(2):E229-36. doi: 10.1152/ajpendo.00409.2012. PubMed PMID: 23193052; PubMed Central PMCID: PMC3543567.
