Description
Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb 11-9 was cloned from mice immunized with diphospho-peptide [CMDSKS(p)S(p)GWGM]. 11-9 recognizes human TDP-43 phosphorylated on serines 409 and 410. 11-9 is validated for western blot, ELISA and IHC(f). This antibody is a powerful tool for biochemical and immunohistochemical analyses of TDP-43 proteinopathy.
TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases.
This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.
Specifications
- Product type primary antibody
- Immunogen CMDSKS(p)S(p)GWGM, S(p): phosphoserine 409/410
- Raised in mouse
- Clone name 11-9
- Isotype IgG1
- Source culture supernatant
- Purification ammonium sulfate precipitation
- Form liquid containing 50% glycerol and 0.05% NaN3
- Volume 50 uL
- Label unlabeled
- Specificity phospho-TDP-43
- Cross reactivity human
- Storage below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.
Recommended dilutions:
- Western blotting 1/1000-1/3000
- Immunohistochemistry 1/3000-1/10000
- ELISA 1/1000-1/5000
- Other applications not tested
- Optimal dilutions/concentrations should be determined by end user.
References
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- Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473
