Description
Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKS(p)SGWGM]. This pAb recognizes human TDP-43 phosphorylated on serine 409 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.
TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.
Specifications
- Product type primary antibody
- Immunogen CMDSKS(p)SGWGM, S(p): phosphoserine 409
- Raised in rabbit
- Source serum
- Form liquid antiserum with 0.05% NaN3 as a preservative
- Volume 100 uL
- Label unlabeled
- Specificity phospho-TDP-43
- Cross reactivity human
- Storage below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.
Recommended dilutions
- Western blotting 1/1000-1/5000
- Immunohistochemistry 1/1000-1/5000
- ELISA 1/1000-1/5000
- Other applications not tested
- Optimal dilutions/concentrations should be determined by end user.
References
- Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473
