Description
Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser410 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKSS(p)GWGM]. This pAb recognizes human TDP-43 phosphorylated on serine 410 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.
TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.
Specifications
- Product type primary antibody
- Immunogen CMDSKSS(p)GWGM, S(p): phosphoserine 410
- Raised in rabbit
- Source serum
- Form liquid antiserum with 0.05% NaN3 as a preservative
- Volume 100 uL
- Label unlabeled
- Specificity phospho-TDP-43
- Cross reactivity human
- Storage below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.
Recommended dilutions
- Western blotting: 1/1000-1/3000
- Immunohistochemistry: 1/1000-1/5000
- ELISA: 1/1000-1/5000
- Other applications not tested
- Optimal dilutions/concentrations should be determined by end user
References
- Hasegawa M, Arai T, Nonaka T, et al. Phosphorylated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Ann Neurol. 2008;64(1):60–70. doi:10.1002/ana.21425
