MOUSE ANTI-CYTOMEGALOVIRUS GLYCOPROTEIN H (GH) (0861)

Mouse anti Cytomegalovirus glycoprotein H antibody (clone 0861) recognises structural glycoprotein H of Cytomegalovirus (CMV). The Cytomegalovirus glycoprotein H antibody does not react with Herpes Simplex Virus, Varicella-zoster virus, Epstein-Barr virus or uninfected cells.

PRODUCT DETAILS – MOUSE ANTI-CYTOMEGALOVIRUS GLYCOPROTEIN H (GH) (0861)

• Mouse anti-CMV Glycoprotein H monoclonal IgG1 antibody (clone 0861).
• Greater than 90% purity by SDS-PAGE and buffered in PBS, pH7.2.

BACKGROUND

Human Cytomegalovirus (HCMV) is an enveloped, double stranded DNA virus that belongs to the family Herpesviridae. The human CMV virus is ubiquitous and HCMV infection is globally widespread. The virus can be transmitted through direct contact with infected bodily fluids or though contact with virus infected transplant and transfusion material. Human CMV can also be transferred in utero from mother to foetus during pregnancy resulting in congenital HCMV infection of the newborn.

Individuals can be re-infected by the same strain or by a different strain of HCMV. Once infected the virus remains latent in an individual and may reactivate later in life. In developed countries, 50 to 70% of all adults are thought to be HCMV sero-positive. Reports suggest that HCMV sero-prevalence increases with age, ethnicity, low socioeconomic status and sexuality.

In healthy adults and children, HCMV infection is self-limiting with most cases being asymptomatic or presenting with mild, non-specific or mononucleosis-like symptoms. However, HCMV can cause severe clinical disease in immunocompromised patients. Congenital HCMV infection can lead to birth defects such as blindness, deafness, mental impairment, epilepsy and microcephaly.

Human CMV has the largest genome of any known human virus. The HCMV genome encodes numerous glycoproteins including structural glycoproteins gB, gH, gL, gO, UL128, UL130 and UL131, which are conserved between members of the family Herpesviridae. Human CMV glycoproteins gB and gH/gL are important for viral entry into most host cells. Receptor-mediated viral entry into endothelial cells requires a functional gH pentameric complex (gH/gL/UL128/UL130/UL131), and this complex is one of the primary targets for antiviral antibodies in infected individuals.

REFERENCES

• Burke HG, Heldwein EE. (2015). Crystal Structure of the Human Cytomegalovirus Glycoprotein B. PLoS Pathog. Oct 20;11(10):e1005227.