APP-BP1 [untagged] / UBA3 [untagged]

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Species Reactivity: human  

Source: Insect sf21  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: APP-BP1 = 60.46 kDa UBA3 = 49.35 kDa  

Protein Sequence: Accession number: APP-BP1 = NP_003896 and UBA3 = NP_003959.3 For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1 Thioester NEDD8 Loading Assay: The activity of APP-BP1/UBA3 was validated by loading NEDD8 onto the active cysteine of APP-BP1/UBA3. Incubation of the APP-BP1/UBA3 enzyme in the presence of NEDD8 and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8 / APP-BP1/UBA3 thioester bond to the reducing agent DTT was confirmed.