ATG7 [6His-tagged]

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Species Reactivity: human  

Source: Insect sf21  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol   

Concentration: 0.5 mg/ml  

Molecular Weight: 81.34 kDa  

Protein Sequence: Accession number: NP_006386. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1 Thioester GABARAP Loading Assay: The activity of His-ATG7 was validated by loading GABARAP onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of GABARAP and ATP at 30 °C was compared at two time points, T0 and T10 minutes. Sensitivity of the GABARAP/His-ATG7 thioester bond to the reducing agent DTT was confirmed.