Description
For PCR Blend Taqî and Blend Taqî -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method(1). This method uses a DNA polymerase which lacked 3'âÂÂ5' exonuclease (proofreading) activity (e.g., TaqDNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a 'mixed' enzyme solution enables highly efficient amplification.
Blend Taqî and Blend Taqî -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
Application: PCR
Features: Effective for the amplification of various targets from small starting template amounts.
The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification.
The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.
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Blend Taqî and Blend Taqî -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.
Application: PCR
Features: Effective for the amplification of various targets from small starting template amounts.
The use of Hot Start technology with anti-Taq DNA polymerase antibodies results in highly efficient amplification.
The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.
