Description
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CARD MEDIUM® is a novel culture medium that acts during IVF on the mouse egg zona pellucida to promote high fertilization rates, especially when used in conjunction with CARD FERTIUP® Preincubation Medium: PM. CARD MEDIUM® was developed by The Center for Animal Resources and Development (CARD), Kumamoto University, Japan.
CARD MEDIUM® is an IVF culture medium that acts on the zona pellucida of the egg during IVF and improves the fertilization rate. Combined use of FERTIUP® sperm cryoprotectant and CARD FERTIUP® Preincubation Medium: PM greatly improves the fertilization rate of frozen-thawed sperm in C57BL/6 and 129 strains, from less than 10% using conventional methods to more than 30%. Further, when coupled with our new CARD MEDIUM®, fertility rates are boosted to over 80%. Thus, combined usage of the trio of CARD FERTIUP®Cryoprotectant: CPA, CARD FERTIUP® Preincubation Medium: PM and CARD MEDIUM® provides a high and consistent rate of fertilization from frozen-thawed mouse sperm, which will allow you to efficiently recover mouse lines when needed.
For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website: http://card.medic.kumamoto-u.ac.jp/card/english/sigen/index.html
