Description
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The kit is designed to measure caspase 3 activity in cell cultures.
Background: BQCkit Caspase-3 Fluorometric Assay Kit provides a simple means for assaying the activity of caspases that recognize the sequence DEVD. The fluorophore 7-amino-4-trifluoromethylcoumarin (AFC) after cleavage from the labeled substrate DEVD-AFC is detected. The AFC can be monitored in a spectrofluorometer at an excitation wavelength of 400 nm and an emission wavelength range of 480-520 nm. Comparison of AFC fluorescence from an apoptotic sample vs AFC fluorescence of control allows determination of the fold increase in caspase-3 activity.
