MOUSE IGG2A ISOTYPE CONTROL ANTIBODY (HOPC-1)

Mouse IgG2a isotype control antibody is supplied as a purified monoclonal antibody for use in immunoassay research and development. The antibody is suitable for use as an isotype matched negative control in a wide range of applications.

PRODUCT DETAILS – MOUSE IGG2A ISOTYPE CONTROL ANTIBODY (HOPC-1)

• Mouse monoclonal IgG2a antibody (clone HOPC-1), suitable for use as a negative control.
• Greater than 95% purity by SDS-PAGE and buffered in borate buffered saline pH8.2.

BACKGROUND

Isotype controls are a type of negative control designed to measure the level of non-specific background signal caused by primary antibodies. The background signal is often the result of immunoglobulins binding non-specifically to Fc receptors present on the cell surface. For example, antibodies raised in mice may bind to some human cells regardless of the test antibody specificity, in a manner related to the isotype of the antibody being used. In these cases you need an isotype control of the same isotype as the test antibody to provide a relevant control for non-specific binding.