Description
Bovine serum albumin (BSA) (50 mg/ml) was incubated at 37°C for 24 h with glyoxylic acid and 100 mM sodium cyanoborohydride in 0.2 M sodium phosphate buffer (pH 7.8), followed by dialysis against PBS. The CML content (2.3 mol CML/mol BSA) was determined by amino acid analysis.
