COS banker [Cell Freezing Medium] (chemical difined)

Cos Banker is a one step culture cell storage solution. Suspend culture cells in Cos Banker and store at -80 degrees for long term storage (it is not necessary to store in liquid nitrogen). This is a serum-free solution with no animal products added.

Feature and Advantages

• Ready to Use (No Dilution Required!)
• Possible to store the Cells at -80℃ in Deep freezer
• Possible to retain Cells in high Viability after defrosting
• Does not contain Serum or Animal products*
• Endotoxin-free tested / Bacteria free tested

   * "Cos Banker II" contains Human Serum Albmin (GMP Grade) as Stabilizer

Components

• Cos Banker (120 ml)
• Contains DMSO 10%
• No serum

Store at 2-10 degrees of dark place
Expiration date: 2 years from date of manufacture

Applications tested

• OKT4 (mouse hybridoma cells)
• PC12 (human neuronal cells)
• K562 (human leukemia cells)
• Other human and mouse lymphocytes

Instructions

1. Collect culture cells (during logarithmic growth stage) and place 5x10⁵ - 1x10⁷ cells in a cryovial. Centrifuge for 5 minutes at 1,200 rpm.
2. Add 1 ml of Cos Banker to cell pellet and vigorously mix, then freeze and store at -80°C.
3. If liquid Nitrogen storage is desired, freeze cells at -80°C first and then transfer to liquid Nitrogen.
4. To thaw, defrost in 37°C water bath quickly.
   Note: It is ideal to use a logarithmic growth stage culture in order to increase the survival rate when thawed.