Description
Purpose of use
Slow freexing of human iPS/ES cells
| Composition | % |
| Hydroxyethyl starch(HES) | 6% |
| Dimethyl sulfoxide(DMSO) | 5% |
| Ethylene glycol | 5% |
| Sodium chloride |
References
1) Imaizumi, Keitaro. et al, : A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. PLoS One. 2014 Feb 12;9(2):e88696.
2) Imaizumi, Keitaro. et al, : A simple and efficient method of slow freezing for human embryonic stem cells and induced pluripotent stem cells. ES Cells: Methods and Protocols-2nd Edition, Springer Protocols, 2015 in press.
