Cul1/Rbx1/Skp1 [untagged]

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Species Reactivity: human  

Source: Insect (Sf21)  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: Cul1=119kDa; Rbx1=12kDa; Skp1=18kDa  

Protein Sequence: Accession number: Cul1=NP_003583.2; Rbx1=NP_055063.1; Skp1= NP_008861.2. For full protein sequence information download the Certificate of Analysis p  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The activity of Cul1/Rbx1/Skp1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase GST-DCNL1 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul1/Rbx1/Skp1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of GST-DCNL1 at 4 °C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul1 subunit in the presence of GST-DCNL1 was demonstrated.