Cul5/Rnf7 [untagged]

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Species Reactivity: human  

Source: Insect (Sf21)  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 0.5 mg/ml  

Molecular Weight: Cul5=121kDa; Rnf7=13kDa  

Protein Sequence: Accession number: Cul5=NP_003469.2; Rnf7=NP_055060.1. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E3 Ligase Assay: The activity of Cul5/Rnf7 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL1 and thioester loaded His-Ube2M-NEDD8. Incubation of Cul5/Rnf7 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL1 at 4 °C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul5 subunit in the presence of His-DCNL1 was demonstrated.