Description
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The kit is designed for reactive oxygen species (ROS) detection in cell cultures by flow cytometer, microscopy or fluorimeter
Background: Cell permeant reagent 2’-7’dichlorofluorescin diacetate (DCFH-DA) is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity. After cell uptake, DCFH-DA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’-7’dichlorofluorescein (DCF). DCF is a fluorescent compound which can be detected by fluorimeter, flow cytometer or fluorescence microscope with a maximum excitation and emission spectra of 495 nm and 529 nm respectively.
