Description
Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.
The kit is designed for the antioxidant capacity measurement in foods, beverages and biological samples.
Background: Bioquochem DMPD assay kit is recommended for antioxidant measurements of beverages. This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms. When the compound N,N-dimethyl-Ï-phenylenediamine (DMPD) is in the presence of a suitable oxidant solution, a colored radical cation is formed (DMPDâ¢+). Antioxidant compounds, which are able to transfer a hydrogen atom to DMPDâ¢+, cause a decoloration of the solution. In our assay a solution of DMPD at an acidic pH and in the presence of a suitable oxidant solution, can form a stable and colored radical cation (DMPDâ¢+) which shows a maximum of absorbance at 553 nm. Antioxidant compounds which are able to transfer a hydrogen atom to DMPDâ¢+ quench the color and produce a decoloration of the solution which is proportional to their amount. This reaction is rapid and the end point, which is stable, is taken as a measure of the antioxidative efficiency.
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