Description
Applications: Functional studies on translesion DNA synthesis by DNA polymerase kappa
Antigen for western blotting and ELISA by anti-Pol kappa antibody Mammalian DNA polymerase K, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis (1). Human DNA polymerase K copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10##-3### and 2 x 10##-3###, respectively. These error rates are high when compared to those of most other DNA polymerases (2). DNA polymerase K has important role in the mutagenic bypass of certain types of DNA lesions (3).
This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase K (1-560 aa), and highly purified by several steps of chromatography (2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
Data Link: U UniProt KB/Swiss-Prot Q9UBT6 (POLK_HUMAN)
Storage: Store at -20ðC
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Antigen for western blotting and ELISA by anti-Pol kappa antibody Mammalian DNA polymerase K, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis (1). Human DNA polymerase K copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10##-3### and 2 x 10##-3###, respectively. These error rates are high when compared to those of most other DNA polymerases (2). DNA polymerase K has important role in the mutagenic bypass of certain types of DNA lesions (3).
This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase K (1-560 aa), and highly purified by several steps of chromatography (2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
Data Link: U UniProt KB/Swiss-Prot Q9UBT6 (POLK_HUMAN)
Storage: Store at -20ðC
