Description
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DnaK Mix is a newly developed supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and improve solubility for proteins that require molecular chaperones for proper folding. It comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE.
DnaK Mix is a newly developed supplement for the PUREfrex® series, designed to assist proper folding and improve solubility of proteins that require molecular chaperones for proper protein folding. DnaK Mix comprises an optimized ratio of highly purified E.coli-derived DnaK, DnaJ and GrpE. ATPase activity of DnaK (Hsp70) is stimulated by co-chaperones DnaJ and GrpE. DnaJ promotes the ATPase activity of DnaK and can bind to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange rate of DnaK.
