Description
Please note that Koma Biotech items have a $300 minimum. Please contact us if you have any questions.
Description: KOMA Biotech launched its new EzWay⢠Mouse Tail Direct PCR Kit, which is designed to enable DNA amplification directly from mouse tail without DNA extraction. Just cut the mouse tail and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. It doesn't require overnight incubation or Proteinase K (PK) treatment as well as DNA purification process. Direct Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl2, dye, additives and Direct PCR Buffer, so you can just add the tail lysate and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional genotyping methods. Also, it reduces risk of handling errors and contamination
Features: Genotyping of transgenic mouse using tail or ear tissueFast and simple method for genotyping without DNA purificationJust 10 minutes incubation requiredNo need to add Proteinase KSaving enormous amounts of material and timeOptimized MasterMix type containing Taq enzyme, dNTP, Direct PCR Buffer, MgCl2, Red dye and additivesDirect loading of PCR products without adding red dyeNo. of Applications : 100 reactions at 20ul PCR volume
Applications: Mouse Tail PCR