Description
Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.
Gallic acid is a polyphenol produced by plants, which shows antioxidant properties. It is commonly used to measure polyphenol concentration but could also be used to measure antioxidant capacity. It is present in a wide variety of plant-based food samples.
RECOMMENDED USES
To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE). It could be used for biological and food samples.
COMPATIBLE BUFFERS
| Reference | Product | Description |
| BQC-ST08006 | Bio-Buffer | 150 mM Sterile PBS pH 7.4 |
| BQC-ST08007 | General Buffer | 100 Mm PBS pH 7 |
| BQC-ST08005 | Nutrition Buffer | 0.1 M PBS pH 5.8 |
STANDARD CALIBRATION
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
|
| Standard (µl) | Diluent (µl) | GAE (µM) |
| 1 | 0 | 1000 | 0 |
| 2 | 10 | 990 | 10 |
| 3 | 25 | 975 | 25 |
| 4 | 50 | 950 | 50 |
| 5 | 75 | 925 | 75 |
| 6 | 100 | 900 | 100 |
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
e-BQC SPECIFICITY
Gallic acid is more predominantly present in Q2 where it has higher sensitivity. It is recommended to use Q2 for calibrations at low concentrations of Gallic acid.
|
| Q1 | Q2 | Qt |
| Sensitivity | 0.02 µC/ µM | 0.05 µC/ µM | 0.8 µC/ µM |
| LOD | 5 µM | 10 µM | 7 µM |
| LOQ | 17 µM | 30 µM | 25 µM |
| LOB | 2 µM | 12 µM | 14 µM |
| RSD | 15 % | 10 % | 14 % |
