Description
See all GenomONE⢠Series Kits for transfection and cell fusion
Please note that there is now only one format of GenomONEâ¢-CF: ISK-CF-001-EX. This format is lyophilized. (The frozen version (ISK-CF-001-FZ) has been discontinued. See below for details*)
For hybridoma preparation and other cell fusion procedures. GenomONEâ¢-CF is a cell fusion kit comprised of Sendai virus envelope (SeV-E, HVJ Envelope, HVJ-E) and special buffers. It can be used with both adhering cells and suspension cells. Fusion of the same or different cell types can be completed in only 30 minutes.
Advantages: GenomONE⢠- CF produces more antibody secreting hybridomas than PEG. (following cell fusion, cells were grown in media containing hybridoma growth supplement as indicated).
Normal BALB/c mouse splenocytes (1x10^8 cells) not sensitized with antigen were fused to X63-Ag8.653 myeloma cells (1x10^7 cells) using GenomONEâ¢-CF EX or PEG1500. The fused cells obtained with each agent were inoculated onto five 96-well plates (Day 0). Beginning the following day, half of the culture medium (10%FBS/RPMI1640) was replaced with HAT medium at five points of time (Days 1, 2, 3, 5, and 8), and the growth of colonies in each well was assessed on Days 10 -11 to determine the hybridoma-positive rate (an indicator of efficiency of fusion). On Day 12, mouse antibody level (IgG + IgA + IgM) in the supernatant was measured by ELISA, to calculate the antibody production-positive rate. The effect of adding a commercially available hybridoma supplement to the medium after fusion was also assessed (supplement was also added to the HAT medium).
Applications (including but not limited to the following examples):
1. Hybridoma preparation: Cell fusion involving PEG and electrofusion, Preparation of monoclonal antibodies, Fusion of antigen-sensitized B cells and myeloma cells
2. Fusion of suspension cells
3. Fusion of adherent cell and suspension cell
4. Application in premature chromosome condensation (PCC): Detection of chromosomal damage and repair by fusion of different types of cells in different cell phases
5. Research on regenerative medicine and cytotherapy: Fusion of somatic cells and stem cells, etc.
6. Research on embryonic development/differentiation: Nuclear transfer (nuclear replacement)
*Originally, ISK-CF-001-EX (lyophilized) was sold world-wide. When we were unable to sell the lyophilized version in the US, we sold an alternate format (ISK-CF-001-FZ) that included a frozen reconstituted virus. Now that we are able to sell ISK-CF-001-EX again, ISK-CF-001-FZ has been discontinued.
