Description
See all GenomONE™ Series Kits for transfection and cell fusion
Please note that there is now only one format of GenomONE™-Neo: ISK-GN-001-EX. This format is lyophilized. (The frozen version (ISK-GN-001-FZ) has been discontinued. See below for details*)
GenomONE™ -Neo is a unique non-viral reagent for transfection that allows for efficient transfection of siRNA, DNA oligonucleotides, and proteins. It involves to use of inactivated Sendai virus: HVJ (hemagglutinating virus of Japan) Envelope VECTOR KIT, a tool for transfection of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion. The HVJ envelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope.
GenomONE™-Neo provides ready-to-use kits containing the lyophilized HVJ envelope and necessary auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer).
Features
- HVJ-E can incorporate various molecules (plasmid DNA, siRNA/miRNA, proteins)
- HVJ-E-incorporated molecules are introduced into cells via membrane fusion
- HVJ-E is also applicable to in vivo transfection
- The original HVJ-E transfection reagent
- Low toxicity with primary cells
- Protocols for in vivo transfection
- Well-cited in the literature
Principle: What is HVJ Envelope (HVJ-E)?
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ#). It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.
It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid atthe terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (another component of the envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potentials in humans or experimental animals. HVJ-E can be used safely at ordinary laboratories, without requiring any special operations or facilities.
Conventional non-viral transfection tools, including cationic lipids, are incorporated into cells through endocytosis which results in degradation of the transferred DNA by lysosomes. On the other hand, HVJ Envelope VECTOR resists degradation by lysosomes, makingit easy to transfer the specified DNA. Therefore, HVJ Envelope VECTOR yields highly efficient gene expression. Sialic acid receptors, which are needed to trigger binding with HN protein, exist in almost all animal cells. Thus, HVJ EnvelopeVECTOR isuseful for a wide range of targets.
Advantages:
1. Wide usability - GenomONE™ HVJ Envelope VECTOR KIT is a highly flexible tool for transfecting a wide variety of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cultured cells (adherent and non-adherent) and tissue. GenomONE™ is useful for transfecting sensitive primary cells and is further distinguished by its ability to deliver contents into live animals. Many literature citations are available for each GenomONE™-Neo application.
2. Safety - Unlike cationic lipids, GenomONE™-Neo delivers the molecule of interest into cells via membrane fusion, not by endocytosis where cargo may be degraded by lysosomal enzymes. Since GenomeONE™-Neo is a purified HVJ product, prepared from virus particles completely inactivated for infectious ability and proliferative potential, it is completely safe to use without special precautions.
3. Ease of use - GenomONE™ provides ready-to-use kits containing the HVJ envelope and required auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, and buffer). Suggested protocols for all major applications are included.
Applications (including but not limited to these examples):
1. siRNA transfection
in vitro: Primary T cell (Human peripheral blood), Primary cardiac myocyte (Rat cardiac myocyte), Differentiated C2C12 (Mouse myoblast), monkey ES cells, Min6 (Mouse pancreatic (BETA) cell), U937 (Human myelomonocytic cell)
in vivo: Intradermally transplanted human cervix cancer /HeLa (SCID mouse), Submandibular gland (rat)
2. Protein delivery
in vitro: Primary macrophage (C3H mouse peritoneal resident), Swiss 3T3 cell (Mouse embryonic fibroblasts)
in vivo: Nucleus tractus solitarius (Rat brain)
3. Oligo DNA transfection
in vitro: Primary HDMECs (Human dermal microvascular endothelial cell), Primary CD34+ cell (Human blood)
in vivo: Uterus (Mouse), Skin (ear lobe(Mouse)), Lung (Mouse)
4. Plasmid DNA transfection
in vivo: Uterus(Mouse), Palatal periodontal tissue (Rat), Myocardium (Rat heart), Lung (neonatal porcine), Subcutaneously transplanted human colon cancer /LoVo (nude mouse)
*Originally, ISK-GN-001-EX (lyophilized) was sold world-wide. When we were unable to sell the lyophilized version in the US, we sold an alternate format (ISK-GN-001-FZ) that included a frozen reconstituted virus. Now that we are able to sell ISK-GN-001-EX again, ISK-GN-001-FZ has been discontinued.
