direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)
