Zeocin, Selective Antibiotic

Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990, a phleomycin D1 formulation, and a selective antibiotic for the Sh ble gene.

Zeocin™ is inserted into DNA and causes apoptosis by cleaving DNA, and it works effectively on aerobic cells. Therefore, Zeocin™ is an antibiotic that can be used to effectively select vectors containing Sh ble genes from various cell types (bacteria, eukaryotic microorganisms, plant cells and animal cells) .

Resistance to Zeocin™ is conferred by the Sh ble gene of Streptoalloteichus hindustanus , and Zeocin™ is inactivated upon binding to antibiotics. The Sh ble gene has a small size of 370bp and is used in various vectors of InvivoGen.

In general, mammalian cells are sensitive to concentrations of 50-400 µg/ml and bacteria at concentrations of 25 µg/ml.

Product concentration:

100 mg/ml in solution

Quality Control:

lot variation tested thoroughly.

Endotoxin level:

< 1 EU/mg

Physicochemical characterization:

HPLC, pH and character

Cell-culture tested:

Zeocin™-sensitive and Zeocin™-resistant tested in mammalian cells.

Non-cytotoxicity of trace contaminants:

It has been confirmed in Zeocin™-resistant cells that there is no problem with long-term use.