Description
Reactivity: Mouse, Rat, Human
This EIA kit is used for quantitative determination of rat, mouse or human pancreatic glucagon in plasma sample. The kit is characterized for sensitive quantification, high specificity and no influence with other components in plasma and needlessness of sample pre-treatment. Glucagon standard used in the kit system is highly purified synthetic product (purity: higher than 98%) and biotinylated pancreatic glucagon is purified by HPLC. Supplementary: Competitive
Applicable Sample: Plasma
Other: According to many studies on glucagon immunoassay, it has been established that the antibody against the C-terminal fragment (19-29) of glucagon has specific binding with pancreaticglucagon, whereas the antibody against the N - terminal fragment (1-19) of glucagon has specific binding with both of pancreatic and intestinal glucagon (total glucagon). Once, 30K by Unger et. al had been widely used as an antibody specific for theC- terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic glucagon specific antibody using synthetic peptide with the Cterminal fragment (19-29) of glucagon as immunogen in 1981. This EIA kit has beendeveloped by using polyclonal antibody against glucagons (19-29), synthetic pancreatic glucagon as glucagon standard and biotinylated pancreatic glucagon as labeled antigen for the measurement of rat, mouse or human glucagon in plasma.
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This EIA kit is used for quantitative determination of rat, mouse or human pancreatic glucagon in plasma sample. The kit is characterized for sensitive quantification, high specificity and no influence with other components in plasma and needlessness of sample pre-treatment. Glucagon standard used in the kit system is highly purified synthetic product (purity: higher than 98%) and biotinylated pancreatic glucagon is purified by HPLC. Supplementary: Competitive
Applicable Sample: Plasma
Other: According to many studies on glucagon immunoassay, it has been established that the antibody against the C-terminal fragment (19-29) of glucagon has specific binding with pancreaticglucagon, whereas the antibody against the N - terminal fragment (1-19) of glucagon has specific binding with both of pancreatic and intestinal glucagon (total glucagon). Once, 30K by Unger et. al had been widely used as an antibody specific for theC- terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic glucagon specific antibody using synthetic peptide with the Cterminal fragment (19-29) of glucagon as immunogen in 1981. This EIA kit has beendeveloped by using polyclonal antibody against glucagons (19-29), synthetic pancreatic glucagon as glucagon standard and biotinylated pancreatic glucagon as labeled antigen for the measurement of rat, mouse or human glucagon in plasma.
