Description
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GroE Mix is a supplement for the PUREfrex® series reconstituted coupled transcription/translation systems, designed to promote proper folding and solubility of proteins that require molecular chaperones for proper protein folding. GroE Mix comprises an optimized ratio of highly purified E.coli-derived GroEL (Hsp60) and GroES.
Chaperone Supplement
DnaK Mix comprises highly purified and ratio-optimized E. coli DnaK, DnaJ and GrpE. DnaK, known as Hsp70, has ATPase activity and is stimulated by co-chaperones DnaJ and GrpE. DnaJ facilitates DnaK ATPase activity and binds to hydrophobic regions of synthesized proteins. GrpE stimulates ADP/ATP exchange of DnaK. DnaK Mix works well with PUREfrex®series and DsbC Set.
Chaperone Supplement
GroE Mix comprises highly purified and ratio-optimized E. coli GroEL (known as Hsp60) and GroES (works in conjunction with GroEL).
| Reagent | Qty | Description | Storage |
| GroE Mix | 12.5 ul | 20µM GroEL, 14-mer form, and 40µM GroES, 7-mer form, from E.coli in 30% glycerol buffer (No tags) | -80 C |
| Dilution Buffer | 500 ul | 30% glycerol buffer | -20 C |
| Reagent | Qty | Description | Storage |
| DnaK Mix | 25 ul | 100µM DnaK, 20µM DnaJ and 20µM GrpE from E.coli in 30% glycerol buffer (No tags) | -80 C |
| Dilution Buffer | 500 ul | 30% glycerol buffer | -20 C |
