Harris Modified, Hematoxylin Solution

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Hematoxylin is frequently used together with Eosin, nuclear fast red, periodic acid or alcian blue. It is also used in immunohistochemical procedures to visualize the end product. Harris Hematoxylin does not contain mercury.

Uses and sample preparation:
Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

Components:


• Harris Hematoxylin


Short procedure:


1. Add Hematoxylin

2. Wash with tap water

3. Add Glacial acetic acid

4. Wash with tap water

5. Add Eosin solution

6. Wash with tap water

7. Dehydrate with graded ethanol, clear and coverslip


Staining characteristics:
Nuclei can be visualized as blue-purple.