Description
Application: DB, ELISA, WB
Clonality: Polyclonal
Applications: It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
It can be used as standard for the quantitative analysis of HIV-1 p15 antigen. HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the bindingof HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA (1). The product is over-expressed as a recombinant protein in E.coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles (Fig 1).
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Clonality: Polyclonal
Applications: It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
It can be used as standard for the quantitative analysis of HIV-1 p15 antigen. HIV-1 Gag p15 is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by the bindingof HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA (1). The product is over-expressed as a recombinant protein in E.coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles (Fig 1).
