Description
Applications: It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
It can be used as standard for the quantitative analysis of HIV-1 p17 antigen.
It can be used in studies of structure and function of AIDS virus as matrix protein that constitutes HIV-1 core. HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitute the essential element of the of AIDS virus particle construction (1). The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography(3). Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles (Fig 1).
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It can be used as standard for the quantitative analysis of HIV-1 p17 antigen.
It can be used in studies of structure and function of AIDS virus as matrix protein that constitutes HIV-1 core. HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitute the essential element of the of AIDS virus particle construction (1). The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography(3). Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles (Fig 1).
