Description
Applications: It is highly effective for the screening of specific inhibitors for new AIDS treatment (3).
Generally, Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However, by using this enzyme in combination as an antigen, the detection will be more sensitive.
Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
Standards for SDS-PAGE (Fig.1), Western blotting (Fig.2), Dot blotting, ELISA. HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1 (AIDS virus), subtype B origin (1). It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus. The product is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified (2). It is composed of two subunits (molecular weight of 66 kD and 51 kD), like the enzyme purified from AIDS virus particles (Fig 1).
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Generally, Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However, by using this enzyme in combination as an antigen, the detection will be more sensitive.
Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
Standards for SDS-PAGE (Fig.1), Western blotting (Fig.2), Dot blotting, ELISA. HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1 (AIDS virus), subtype B origin (1). It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus. The product is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified (2). It is composed of two subunits (molecular weight of 66 kD and 51 kD), like the enzyme purified from AIDS virus particles (Fig 1).
