ISG15 [untagged]

Customer Notice Regarding Orders for Ubiquigent Products: From September 2020, orders for Ubiquigent products must total at least $700 to enable prompt delivery. Orders less than this amount may be subject to unpredictable wait times. Please contact us for further details.

Species Reactivity: human  

Source: E.coli  

Formula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol  

Concentration: 1 mg/ml  

Molecular Weight: ~17.59 kDa  

Protein Sequence: Accession number: NP_005092. For full protein sequence information download the Certificate of Analysis pdf.  

Quality Control Protein Identification: Confirmed by mass spectrometry.  

Quality Control Activity: E1 Thioester ISG15 Loading Assay: The activity of ISG15 was validated by loading ISG15 onto the active cysteine of His-UBA7. Incubation of the His-UBA7 enzyme in the presence of ISG15 and ATP at 30 °C was compared at two time points, T0 and T10 minutes.   Sensitivity of the ISG15/His-UBA7 thioester bond to the reducing agent DTT was confirmed.