Lactose Broth

Purpose of Use

• Detection of coliform bacterium
• Test for bacterial lactose resolution

Composition (1L＝20.0g)

• Peptone：10.0g
• Lactose：5.0g
• Meat extracts （BOVINE）：5.0g
• Bromothymol blue：0.024g
• pH：7.2±

Note: Please store at room temperature in a dark place with sealing hermetically.

Procedures

1.  Add 20.0 g of Lactose Broth to 1000 ml of purified water and mix enough.
2.  After heating dissolution, aliquot the solution to the each test tube by 10 ml which was put fermentation tube or Durham pipe in.
3.  Sterilize at high pressure steamof 121 degrees Celsius for 15 minutes. Use it after quenching.
4.  Use it for the inspection of food and an underwater coliform bacterium.

Don't use if air bubbles are left to fermentation tube or the Durham pipe after sterilization.