Description
Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.
The kit is designed for protein quantification in biological samples.
Background: Lowry Protein Quantification Assay is based on Lowry method1, first described in 1951. The method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. This is called a Biuret chromophore and is commonly stabilized by the addition of tartrate2. The second reaction is reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate), primarily by the reduced copper-amide bond complex as well as by tyrosine, tryptohan, histidine, cystine, and cysteine residues in protein3. The monovalent copper ion catalyzes the latter reaction. The reduced Folin-Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500 to 750 nm. The Biuret reaction itself is not very sensitive.
Custom Assay Service Available! Please contact us for more information.
