Description
Lymphoprep™ Tube is a sterile tube in which the Lymphoprep™ is contained below a plastic filter disc (barrier frit). This allows blood to be poured simply and directly into the tube, the disc preventing any mixing with the separation medium. The erythrocytes displace the Lymphoprep™ upwards to allow mononuclear cells to band above the filter disc.
Lymphoprep™ is a ready-made, sterile, and endotoxin tested solution that has been used to isolate mononuclear cells for more than 35 years. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an iso-osmotic medium with a density of 1.077 g/ml. Mononuclear cells are therefore retained at the sample/medium interface while erythrocytes and PMNs sediment through the medium.This method is rapid, simple, and reliable and gives excellent results with blood samples from normal individuals and patients.
Why paque more?
Lymphoprep™ has the same specifications as Ficoll(R)-Paque and Histopaque(R)-1077 and is manufactured to essentially equivalent quality standards. As compared to the top brands, you can save up to 50% on your gradient medium.
Totally tubular
Optimal success of the standard method for isolating mononuclear cells using Lymphoprep™ depends to a large extent on the careful layering of the diluted blood sample on top of the centrifugation medium to maintain a sharp interface between the two layers. This procedure requires some practice and can be time-consuming with large numbers of samples. Take some of the variability out of your experiments by using Lymphoprep™ Tube.
Lymphoprep™ specifications:
- Sodium diatrizoate: 9.1% (w/v)
- Polysaccharide: 5.7% (w/v)
- Density: 1.077 ± 0.001 g/mL
- Osmolality: 290 ± 15 mOsm
- Endotoxins: <1.0 EU/mL
Lymphoprep™ is the ideal medium for:
- PBMC (peripheral blood mononuclear cells), notably monocytes and leukocytes
Product Downloads
- Lymphoprep™ Tube Package Insert
- Lymphoprep™ Tube Leaflet
- Lymphoprep™ Tube MSDS
- Purification of mononuclear cells, monocytes and polymorphonuclear leukocytes
- Lymphoprep™, Isolation of human mononuclear cells
- Lymphoprep™ Tube, Isolation of human mononuclear cells
- Mini-Reviews
- Product Certificates
- Educational Slide Shows
About Lymphoprep
A simple and effective method for the isolation of mononuclear cells from human blood was reported by Dr. Arne Bøyum in 1968. For more than 35 years a commercial medium known as Lymphoprep™ has been widely used for isolating these cells. Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than the erythrocytes and the polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities below 1.077 g/ml. These cells can therefore be isolated by centrifugation on an isoosmotic medium with a density of 1.077 g/ml, which allows the erythrocytes and the PMNs to sediment through the medium while retaining the mononuclear cells at the sample/medium interface.
The described method is rapid, simple and reliable and gives excellent results with blood samples from normal individuals and patients. To obtain the maximum yield it is important that the blood sample is diluted 1:1 with physiological saline before being applied to the Lymphoprep™ The contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total cell number. Some immature PMNs may band with the lymphocytes during intense immunosuppressive therapy. It is essential to remove most of the platelets from mononuclear cell preparations in order to avoid inhibition in the cytotoxicity test.
Lymphoprep™ has the same specifications as the expensive PLUS or PREMIUM media from other manufacturers. Lymphoprep™ is a ready-made, sterile and endotoxin tested solution with the following specifications:
- Sodium diatrizoate: 9.1% (w/v)
- Polysaccharide: 5.7% (w/v)
- Density: 1.077 ± 0.001 g/ml
- Osmolality: 290 ± 15 mOsm
- Endotoxins: < 1.0 EU/ml
Each batch of Lymphoprep™ is checked on the level of endotoxins using a specific LAL test. Axis-Shield's goal is to produce batches with an endotoxin level lower or equal to 0.13 EU/ml. Sterility is claimed according to Ph.Eur.
Lymphoprep™ is manufactured, packed and released in compliance with:
- Current EU Guide to Good Manufacturing Practice
- Fresenius Kabi AS Quality System
- Fresenius Kabi AS Manufacturing License
For every lot, a Certificate of Analysis showing the actual values of density, osmolality and endotoxins is made available at www.axis-shield-density-gradient -media.com.
Lymphoprep™ can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera and immunological research. Thorsby and Brattelie used this technique with only slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.
