MagExtractor-Plasmid-

MagExtractor-Plasmid- is a magnetic silica bead-based kit for rapid, simple and reliable purification of plasmid DNA from E. coli cells. Requiring a suitable magnetic separation stand such as the Magical Trapper (TYB-MGS-101), it exploits the property of silica surfaces to bind DNA in the presence of chaotropic agents (reference 1). Purified plasmids can be used directly in automated fluorescent sequencing.

Features


• ~3-6 ug plasmid yield from E. coli carrying a high-copy plasmid

• Suitable for high throughput extraction of plasmid from E. coli cells

• 10–15-minute extraction time

• Purified plasmid can be directly utilized in sequencing, enzyme reactions, transformation, etc

• Does not contain hazardous substances, such as phenol or chloroform


Contents of kit (sufficient for 500 preparations)


• Binding Solution                     130 ml x 2 (store at 4°C or room temperature)

• Magnetic Beads I                    16 ml (store at 4°C or room temperature)

• Magnetic Beads II                  16 ml (store at 4°C or room temperature)

• Suspension Solution               80 ml (store at 4°C)

• Lysis Solution I                      80 ml (store at 4°C or room temperature)

• Lysis Solution II                     20 ml (store at 4°C)

• Neutralization Solution          65 ml (store at 4°C or room temperature)

• Elution Solution                      60 ml (store at 4°C)

• 5x Loading Dye                      11 ml (store at 4°C)


Caution (Take appropriate laboratory safety measures and wear gloves when handling)


• Binding Solution contains chaotropic salt, an irritant.

• Lysis solution II contains NaOH.

• Suspension Solution contains RNase A.


Notes


• Magnetic Beads I are not necessary for manual use. [This reagent is used for extraction with the automated nucleic acid purification apparatus “MFX series”]

• Lysis solution I and II should be mixed at a ratio of 4:1 prior to use. This solution can be stored at room temperature for 3 weeks.

• Precipitates formed in “Lysis Solution I” at low temperature can be dissolved by heating at 40°C.

• Purified plasmid may contain small amounts of EtOH. Plasmid DNA solutions containing EtOH sink easily into agarose gel slots using 5x Loading Dye.


References


1. B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)