Description
Please note that BioQuochem items have a $300 minimum. Please contact us if you have any questions.
Antioxidant Standards mix (Ascorbic Acid, Galic Acid and TROLOX) : 3 x 1 ml
RECOMMENDED USES
To compare the e-BQC results (µC) to classical antioxidant capacity units of Gallic acid Antioxidant Capacity Equivalents (GAE), Ascorbic Acid (CEAC) or TROLOX (TEAC). It could be used for biological and food samples.
COMPATIBLE BUFFERS
| Reference | Product | Description |
| BQC-ST08006 | Bio-Buffer | 150 mM Sterile PBS pH 7.4 |
| BQC-ST08007 | General Buffer | 100 Mm PBS pH 7 |
| BQC-ST08005 | Nutrition Buffer | 0.1 M PBS pH 5.8 |
STANDARD CALIBRATION
Add 1 ml of the selected diluent buffer to the vial (10 mM). Transfer the 1 ml from the vial and add 9 ml of diluent (1 mM). Prepare the calibration curves in 1.5 ml tubes as shown below:
|
| Standard (µl) | Diluent (µl) | Antioxidant Equivalents (µM) |
| 1 | 0 | 1000 | 0 |
| 2 | 10 | 990 | 10 |
| 3 | 25 | 975 | 25 |
| 4 | 50 | 950 | 50 |
| 5 | 75 | 925 | 75 |
| 6 | 100 | 900 | 100 |
*This is just a recommendation in case the expected antioxidant capacity values of the sample were unknow. You are free to adapt the concentrations to be tested to your necessities.
COMPARISON WITH TAC ASSAY KITS
If a comparison of the e-BQC results with classical antioxidant capacity assays is desired, the following BQC kits that are compatible with a TROLOX standard could be used:
| Product | Reference |
| DMPD | |
| FRAP | |
| FAST FRAP | |
| ORAC | |
| CUPRAC |
